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- Title
Determination of optimal growth parameters for the bioincising fungus Physisporinus vitreus by means of response surface methodology.
- Authors
Schubert, M.; Dengler, V.; Mourad, S.; Schwarze, F. W. M. R.
- Abstract
Aim: To evaluate the influence of water activity ( aw), temperature and pH on the radial growth and lag phase of Physisporinus vitreus (E-642), a basidiomycete was used in the biotechnological process of bioincising. Methods and Results: Radial growth was monitored for 20 days on malt extract agar medium. Five levels of aw (0·998, 0·982, 0·955, 0·928, 0·892) were combined with three incubation temperatures (10, 15, 20°C) and three pH values (4, 5, 6). Data analyses showed a highly significant effect of aw and temperature ( P < 0·0001) and a significant effect of pH ( P < 0·05). The radial growth rate and lag phase of P. vitreus were very sensitive to aw reduction. Although P. vitreus was able to grow at all the selected temperatures and pH values, the lag phase increased with decreasing aw and growth became inhibited at aw = 0·955. Optimal conditions for growth of P. vitreus were aw = 0·998, 20°C and pH 5. The response surface model provided reliable estimates of these growth parameters and confirmed a greater dependence on aw than on temperature or pH under in vitro conditions. Conclusions: Low levels of aw can prevent growth of P. vitreus, so wood moisture content should be adjusted accordingly. Significance and Impact of the Study: Implementation of these results should contribute towards the optimization and efficiency of bioincising.
- Subjects
FUNGI; TEMPERATURE; WOOD preservation &; treatment industry; HYDROPHOBIC surfaces; GROWTH rate; RECOMBINANT DNA; MALT-extracts
- Publication
Journal of Applied Microbiology, 2009, Vol 106, Issue 5, p1734
- ISSN
1364-5072
- Publication type
Article
- DOI
10.1111/j.1365-2672.2008.04138.x