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- Title
Heat-inducible SlWRKY3 confers thermotolerance by activating the SlGRXS1 gene cluster in tomato.
- Authors
Ying Wang; Wenxian Gai; Liangdan Yuan; Lele Shang; Fangman Li; Zhao Gong; Pingfei Ge; Yaru Wang; Jinbao Tao; Xingyu Zhang; Haiqiang Dong; Yuyang Zhang
- Abstract
High temperature stress is one of the major environmental factors that affect the growth and development of plants. Although WRKY transcription factors play a critical role in stress responses, there are few studies on the regulation of heat stress by WRKY transcription factors, especially in tomato. Here, we identified a group I WRKY transcription factor, SlWRKY3, involved in thermotolerance in tomato. First, SlWRKY3 was induced and upregulated under heat stress. Accordingly, overexpression of SlWRKY3 led to an increase, whereas knock-out of SlWRKY3 resulted in decreased tolerance to heat stress. Overexpression of SlWRKY3 accumulated less reactive oxygen species (ROS), whereas knock-out of SlWRKY3 accumulated more ROS under heat stress. This indicated that SlWRKY3 positively regulates heat stress in tomato. In addition, SlWRKY3 activated the expression of a range of abiotic stress-responsive genes involved in ROS scavenging, such as a SlGRXS1 gene cluster. Further analysis showed that SlWRKY3 can bind to the promoters of the SlGRXS1 gene cluster and activate their expression. Collectively, these results imply that SlWRKY3 is a positive regulator of thermotolerance through direct binding to the promoters of the SlGRXS1 gene cluster and activating their expression and ROS scavenging.
- Subjects
PLANT development; TOMATO genetics; PLANT growth; EFFECT of temperature on plants; GENE expression in plants
- Publication
Horticulture Plant Journal, 2024, Vol 10, Issue 2, p515
- ISSN
2095-9885
- Publication type
Article
- DOI
10.1016/j.hpj.2022.12.006