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- Title
Labrenzia sp. BM1: A Quorum Quenching Bacterium That Degrades N-acyl Homoserine Lactones via Lactonase Activity.
- Authors
Ab Ghani, Norshazliza; Norizan, Siti Nur Maisarah; Xin Yue Chan; Wai-Fong Yin; Kok-Gan Chan
- Abstract
We report the degradation of quorum sensing N-acylhomoserine lactone molecules by a bacterium isolated from a Malaysian marine water sample. MALDI-TOF and phylogenetic analysis indicated this isolate BM1 clustered closely to Labrenzia sp. The quorum quenching activity of this isolate was confirmed by using a series of bioassays and rapid resolution liquid chromatography analysis. Labrenzia sp. degraded a wide range of N-acylhomoserine lactones namely N-(3-hexanoyl)-L-homoserine lactone (C6-HSL), N-(3-oxohexanoyl)-L-homoserine lactone (3-oxo-C6-HSL) and N-(3-hydroxyhexanoyl)-Lhomoserine lactone (3-hydroxy-C6-HSL). Re-lactonisation bioassays confirmed Labrenzia sp. BM1 degraded these signalling molecules efficiently via lactonase activity. To the best of our knowledge, this is the first documentation of a Labrenzia sp. capable of degrading N-acylhomoserine lactones and confirmation of its lactonase-based mechanism of action.
- Subjects
SERINE; LACTONES; PHYLOGENY; QUORUM sensing; LIQUID chromatography
- Publication
Sensors (14248220), 2014, Vol 14, Issue 7, p11760
- ISSN
1424-8220
- Publication type
Article
- DOI
10.3390/s140711760