We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Reoxygenation Modulates the Adverse Effects of Hypoxia on Wound Repair.
- Authors
Bai, Que; Gao, Qian; Hu, Fangfang; Zheng, Caiyun; Sun, Na; Chen, Wenting; Liu, Jinxi; Zhang, Yanni; Wu, Xianglong; Lu, Tingli
- Abstract
Hypoxia is a major stressor and a prominent feature of pathological conditions, such as bacterial infections, inflammation, wounds, and cardiovascular defects. In this study, we investigated whether reoxygenation has a protective effect against hypoxia-induced acute injury and burn using the C57BL/6 mouse model. C57BL/6 mice were exposed to hypoxia and treated with both acute and burn injuries and were in hypoxia until wound healing. Next, C57BL/6 mice were exposed to hypoxia for three days and then transferred to normoxic conditions for reoxygenation until wound healing. Finally, skin wound tissue was collected to analyze healing-related markers, such as inflammation, vascularization, and collagen. Hypoxia significantly increased inflammatory cell infiltration and decreased vascular and collagen production, and reoxygenation notably attenuated hypoxia-induced infiltration of inflammatory cells, upregulation of pro-inflammatory cytokine levels (IL-6 and TNF-α) in the wound, and remission of inflammation in the wound. Immunofluorescence analysis showed that reoxygenation increased the expression of the angiogenic factor α-SMA and decreased ROS expression in burn tissues compared to hypoxia-treated animals. Moreover, further analysis by qPCR showed that reoxygenation could alleviate the expression of hypoxic-induced inflammatory markers (IL-6 and TNF), increase angiogenesis (SMA) and collagen synthesis (Col I), and thus promote wound healing. It is suggested that oxygen can be further evaluated in combination with oxygen-releasing materials as a supplementary therapy for patients with chronic hypoxic wounds.
- Subjects
WOUND healing; HYPOXEMIA; VASCULAR endothelial growth factors; LABORATORY mice; CHRONIC wounds &; injuries; BACTERIAL diseases
- Publication
International Journal of Molecular Sciences, 2022, Vol 23, Issue 24, p15832
- ISSN
1661-6596
- Publication type
Article
- DOI
10.3390/ijms232415832