We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Truncated Analogues of a G-Quadruplex-Forming Aptamer Targeting Mutant Huntingtin: Shorter Is Better!
- Authors
Riccardi, Claudia; D'Aria, Federica; Fasano, Dominga; Digilio, Filomena Anna; Carillo, Maria Rosaria; Amato, Jussara; De Rosa, Laura; Paladino, Simona; Melone, Mariarosa Anna Beatrice; Montesarchio, Daniela; Giancola, Concetta
- Abstract
Two analogues of the MS3 aptamer, which was previously shown to have an exquisite capability to selectively bind and modulate the activity of mutant huntingtin (mHTT), have been here designed and evaluated in their physicochemical and biological properties. Featured by a distinctive propensity to form complex G-quadruplex structures, including large multimeric aggregates, the original 36-mer MS3 has been truncated to give a 33-mer (here named MS3-33) and a 17-mer (here named MS3-17). A combined use of different techniques (UV, CD, DSC, gel electrophoresis) allowed a detailed physicochemical characterization of these novel G-quadruplex-forming aptamers, tested in vitro on SH-SY5Y cells and in vivo on a Drosophila Huntington's disease model, in which these shorter MS3-derived oligonucleotides proved to have improved bioactivity in comparison with the parent aptamer.
- Subjects
HUNTINGTON disease; APTAMERS; GEL electrophoresis; MEDICAL model; DIELECTROPHORESIS
- Publication
International Journal of Molecular Sciences, 2022, Vol 23, Issue 20, p12412
- ISSN
1661-6596
- Publication type
Article
- DOI
10.3390/ijms232012412