We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Discovery of Long Non-Coding RNA MALAT1 Amplification in Precancerous Colorectal Lesions.
- Authors
Siskova, Anna; Kral, Jan; Drabova, Jana; Cervena, Klara; Tomasova, Kristyna; Jungwirth, Jiri; Hucl, Tomas; Kohout, Pavel; Summerova, Sandra; Vodickova, Ludmila; Vodicka, Pavel; Vymetalkova, Veronika
- Abstract
A colorectal adenoma, an aberrantly growing tissue, arises from the intestinal epithelium and is considered as precursor of colorectal cancer (CRC). In this study, we investigated structural and numerical chromosomal aberrations in adenomas, hypothesizing that chromosomal instability (CIN) occurs early in adenomas. We applied array comparative genomic hybridization (aCGH) to fresh frozen colorectal adenomas and their adjacent mucosa from 16 patients who underwent colonoscopy examination. In our study, histologically similar colorectal adenomas showed wide variability in chromosomal instability. Based on the obtained results, we further stratified patients into four distinct groups. The first group showed the gain of MALAT1 and TALAM1, long non-coding RNAs (lncRNAs). The second group involved patients with numerous microdeletions. The third group consisted of patients with a disrupted karyotype. The fourth group of patients did not show any CIN in adenomas. Overall, we identified frequent losses in genes, such as TSC2, COL1A1, NOTCH1, MIR4673, and GNAS, and gene gain containing MALAT1 and TALAM1. Since long non-coding RNA MALAT1 is associated with cancer cell metastasis and migration, its gene amplification represents an important event for adenoma development.
- Subjects
LINCRNA; ADENOMATOUS polyps; PRECANCEROUS conditions; COMPARATIVE genomic hybridization; CANCER cell migration; CHROMOSOME abnormalities
- Publication
International Journal of Molecular Sciences, 2022, Vol 23, Issue 14, p7656
- ISSN
1661-6596
- Publication type
Article
- DOI
10.3390/ijms23147656