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- Title
Pre-Ischemic Treadmill Training for Prevention of Ischemic Brain Injury via Regulation of Glutamate and Its Transporter GLT-1.
- Authors
Xiaojiao Yang; Zhijie He; Qi Zhang; Yi Wu; Yongshan Hu; Xiaolou Wang; Mingfen Li; Zhiyuan Wu; Zhenzhen Guo; Jingchun Guo; Jie Jia
- Abstract
Pre-ischemic treadmill training exerts cerebral protection in the prevention of cerebral ischemia by alleviating neurotoxicity induced by excessive glutamate release following ischemic stroke. However, the underlying mechanism of this process remains unclear. Cerebral ischemia-reperfusion injury was observed in a rat model after 2 weeks of pre-ischemic treadmill training. Cerebrospinal fluid was collected using the microdialysis sampling method, and the concentration of glutamate was determined every 40 min from the beginning of ischemia to 4 h after reperfusion with high-performance liquid chromatography (HPLC)-fluorescence detection. At 3, 12, 24, and 48 h after ischemia, the expression of the glutamate transporter-1 (GLT-1) protein in brain tissues was determined by Western blot respectively. The effect of pre-ischemic treadmill training on glutamate concentration and GLT-1 expression after cerebral ischemia in rats along with changes in neurobehavioral score and cerebral infarct volume after 24 h ischemia yields critical information necessary to understand the protection mechanism exhibited by pre-ischemic treadmill training. The results demonstrated that pre-ischemic treadmill training up-regulates GLT-1 expression, decreases extracellular glutamate concentration, reduces cerebral infarct volume, and improves neurobehavioral score. Pre-ischemic treadmill training is likely to induce neuroprotection after cerebral ischemia by regulating GLT-1 expression, which results in re-uptake of excessive glutamate.
- Subjects
BRAIN injury prevention; CEREBRAL ischemia; GLUTAMATE transporters; NEUROTOXICOLOGY; REPERFUSION injury; HIGH performance liquid chromatography; GENE expression; WESTERN immunoblotting
- Publication
International Journal of Molecular Sciences, 2012, Vol 13, Issue 8, p9447
- ISSN
1661-6596
- Publication type
Article
- DOI
10.3390/ijms13089447