We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Immunological response in mice bearing LM3 breast tumor undergoing Pulchellin treatment.
- Authors
Cordeiro de Matos, Djamile; Carolina Abreu de Ribeiro, Lívia; Tansini, Aline; Souza Ferreira, Lucas; Campos Polesi Placeres, Marisa; Luis Colombo, Lucas; Zeppone Carlos, Iracilda
- Abstract
Background: Ribosome-inactivating proteins (RIP) have been studied in the search for toxins that could be used as immunotoxins for cancer treatment. Pulchellin, a type 2 RIP, is suggested to induce immune responses that have a role in controlling cancer. Methods: The percentage of dendritic cells and CD4+ and CD8+ T cells in the spleen (flow cytometry), cytokines' release by PECs and splenocytes (ELISA) and nitric oxide production by PECs (Griess assay) were determined from tumor-bearing mice injected intratumorally with 0.1 ml of pulchellin at 0.75 μg/kg of body weight. Statistical analysis was performed by one-way ANOVA with Tukey's post hoc test. Results: Pulchellin-treated mice showed significant immune system activation, characterized by increased release of IFN-γ and Th2 cytokines (IL-4 and IL-10), while IL-6 and TGF-β levels were decreased. There was also an increase in macrophage's activation, as denoted by the higher percentage of macrophages expressing adhesion and costimulatory molecules (CD54 and CD80, respectively). Conclusions: Our results suggest that pulchellin is promising as an adjuvant in breast cancer treatment.
- Subjects
BREAST tumor prevention; PHYTOCHEMICALS; ANALYSIS of variance; ANIMAL experimentation; BREAST tumors; CELL culture; COMBINED modality therapy; CYTOKINES; DENDRITIC cells; ENZYME-linked immunosorbent assay; FLOW cytometry; IMMUNE system; IMMUNOASSAY; MACROPHAGES; MEDICINAL plants; MICE; NITRIC oxide; HEALTH outcome assessment; SEEDS; STATISTICS; T cells; DATA analysis; TREATMENT effectiveness; DATA analysis software; THERAPEUTICS
- Publication
BMC Complementary & Alternative Medicine, 2012, Vol 12, Issue 1, p107
- ISSN
1472-6882
- Publication type
Article
- DOI
10.1186/1472-6882-12-107