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- Title
Therapeutic potential of natural products and antibiotics against bovine mastitis pathogen of cows and buffaloes.
- Authors
Khan, Wajid; Khan, Shahab Ahmad; Khan, Fayaz Akhtar; Khan, Sohail; Ullah, Ikram; Shah, Abdullah; Uddin, Ihsan; Khan, Haji; Uddin, Muhammad Nazir; Akbar, Fazal; Qayum, Nabila
- Abstract
The present study aims to evaluate the prevalence and antimicrobial sensitivity of Staphylococcus aureus associated with bovine mastitis to selected antibiotics and plant extracts. In the current study, 140 milk sam)ples were collected from cows and buffaloes. Among the 140 samples, 93 samples were positive for sub-clinical mastitis based on the California Mastitis Test (CMT). Out of the total positive samples, 45 were confirmed for S. aureus on a Mannitol salt agar media. The antimicrobial susceptibility test revealed that 44.82% of the isolates were resistant to cefoxitin (oxacillin) confirming methicillin-resistant S. aureus (MRSA) with a higher percentage (51.61%) in the buffalo than in the cow samples. Furthermore, the PCR assay confirmed the presence of the mecA gene in all the MRSA isolates. Among the seven tested antibiotics, sulfamethoxazole + trimethoprim showed high efficacy (71.1%) against methicillin-susceptible S. aureus isolates (MSSA). Oxytetracycline and sulfamethoxazole + trimethoprim showed 20% efficacy against MRSA followed by enrofloxacin (10%). On the other hand, the tested samples from Pistacia chinensis revealed that the ethyl acetate extract of bark showed a maximum zone of inhi)bition of 21.3 mm against MSSA and MRSA isolates at 3 000 µg/disc. Moreover, the methanol extract of Coto)neaster microphyllus formed a 12.3 mm and 9.1 mm zone of inhibition against the MSSA and MRSA isolates, respectively.
- Subjects
BOVINE mastitis; NATURAL products; ANTIBIOTICS; MICROBIAL sensitivity tests; STAPHYLOCOCCUS aureus
- Publication
Veterinární Medicína, 2023, Vol 68, Issue 7, p271
- ISSN
0375-8427
- Publication type
Article
- DOI
10.17221/80/2022-VETMED