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- Title
Detection of antibodies against Leishmania species using enzyme-linked immunosorbent assay in cats from the western border of Brazil.
- Authors
Pradella, Gabriela Döwich; Acunha Escobar, Taiane; dos Santos, Thália Pacheco; Minuzzi, Jennifer Stello; Rosa e Silva, Lívia Kmetzsch; Roman, Isac Junior; Flôres Vogel, Fernanda Silveira; Acosta Duarte, Claudia; Lübeck, Irina
- Abstract
Feline leishmaniosis is infrequent worldwide, and cats have been suggested as secondary reservoirs for the parasite. However, specific diagnostic techniques for feline samples are scarce. In this study, we standardized an in-house indirect enzyme-linked immunosorbent assay (ELISA) using crude Leishmania infantum antigen to detect antibodies in feline samples from an endemic canine visceral leishmaniosis (CVL) area in the western border of Brazil. The results were compared with those of an indirect immunofluorescence assay (IFA). We tested semi-domiciled felines residing in Uruguaiana and Barra do Quaraí, Rio Grande do Sul. Among the 41 samples, 25 (61%) were positive using ELISA and 24 (58%) were positive using IFA (1:40). Our findings demonstrated a high seropositivity of feline samples from the endemic CVL area in the western border of Brazil, and we proposed the use of an in-house ELISA with crude antigen for population screening. This is the first serological survey on felines in a region where CVL is well established.
- Subjects
BRAZIL; RIO Grande do Sul (Brazil : State); ENZYME-linked immunosorbent assay; LEISHMANIA; IMMUNOGLOBULINS; CATS; LEISHMANIASIS
- Publication
Ciência Rural, 2024, Vol 54, Issue 8, p1
- ISSN
0103-8478
- Publication type
Article
- DOI
10.1590/0103-8478cr20230127