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- Title
Comparison of antioxidant enzymes in saliva of elderly smokers and non-smokers.
- Authors
Kanehira, Takashi; Shibata, Kouichi; Kashiwazaki, Haruhiko; Inoue, Nobuo; Morita, Manabu
- Abstract
Objectives: This study was designed to compare the levels of copper/zinc superoxide dismutase (Cu/Zn SOD), peroxidase (POx) and glutathione peroxidase (GSH-Px) in saliva of smokers and those in saliva of non-smokers. Methods: Unstimulated saliva samples were collected from 88 elderly males (65 years old or over) who visited a private dental clinic. Forty-four subjects were current smokers (more than 20 cigarettes daily for at least 30 years) and 44 were non-smokers. The levels of salivary thiocyanate, Cu/Zn SOD, GSH-Px, and POx activity were measured using standard procedures. Results: The mean levels of salivary thiocyanate (SCN−) and SOD were significantly higher ( p < 0.01) in the smoking group than in the non-smoking group, whereas the specific activity levels of POx and GSH-Px were significantly higher ( p < 0.05) in the non-smoking group than in the smoking group. Significant correlation coefficients were found between the levels of SCN− and SOD ( r = 0.37, p < 0.001). In the non-smoking group, a significant positive association was found between specific activity of POx and age ( r = 0.33, p < 0.05). Conclusion: Measurement of SCN− and Cu/Zn SOD in human saliva might be useful for estimating the level of oxidative stress caused by cigarette smoke. Despite increased H2O2 level as a defense system induced by SOD, detoxification of H2O2 might be deteriorated in the oral cavity of elderly smokers.
- Subjects
SMOKING; SALIVA; ANTIOXIDANTS; ORAL cancer; OLDER people
- Publication
Gerodontology, 2006, Vol 23, Issue 1, p38
- ISSN
0734-0664
- Publication type
Article
- DOI
10.1111/j.1741-2358.2006.00077.x