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- Title
Effects of aluminum on the reduction of neural stem cells, proliferating cells, and differentiating neuroblasts in the dentate gyrus of D-galactose-treated mice via increasing oxidative stress.
- Authors
Sung Min Nam; Jong Whi Kim; Dae Young Yoo; Woosuk Kim; Hyo Young Jung; Jung Hoon Choi; In Koo Hwang; Je Kyung Seong; Yeo Sung Yoon
- Abstract
Aluminum (Al) accumulation increases with aging, and long-term exposure to Al is regarded as a risk factor for Alzheimer's disease. In this study, we investigated the effects of Al and/or D-galactose on neural stem cells, proliferating cells, differentiating neuroblasts, and mature neurons in the hippocampal dentate gyrus. AlCl3 (40 mg/kg/day) was intraperitoneally administered to C57BL/6J mice for 4 weeks. In addition, vehicle (physiological saline) or D-galactose (100 mg/kg) was subcutaneously injected to these mice immediately after AlCl3 treatment. Neural stem cells, proliferating cells, differentiating neuroblasts, and mature neurons were detected using the relevant marker for each cell type, including nestin, Ki67, doublecortin, and NeuN, respectively, via immunohistochemistry. Subchronic (4 weeks) exposure to Al in mice reduced neural stem cells, proliferating cells, and differentiating neuroblasts without causing any changes to mature neurons. This Al-induced reduction effect was exacerbated in D-galactose-treated mice compared to vehicle-treated adult mice. Moreover, exposure to Al enhanced lipid peroxidation in the hippocampus and expression of antioxidants such as Cu, Zn- and Mn-superoxide dismutase in D-galactose-treated mice. These results suggest that Al accelerates the reduction of neural stem cells, proliferating cells, and differentiating neuroblasts in D-galactose-treated mice via oxidative stress, without inducing loss in mature neurons.
- Subjects
NEURAL stem cells; PHYSIOLOGICAL effects of aluminum; CELL proliferation; CELL differentiation; DENTATE gyrus; GALACTOSE; OXIDATIVE stress; LABORATORY mice
- Publication
Journal of Veterinary Science, 2016, Vol 17, Issue 2, p127
- ISSN
1229-845X
- Publication type
Article
- DOI
10.4142/jvs.2016.17.2.127