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- Title
P-glycoprotein expression in human oesophageal squamous carcinoma cell lines.
- Authors
Zampieri, L.; Veale, R. B.
- Abstract
Several tumour types show resistance to a broad spectrum of anti-cancer drugs and are referred to as expressing a multidrug-resistant phenotype. This has been associated with the over-expression of P-glycoprotein, a highly conserved transmembrane protein. Increased levels of P-glycoprotein are generated in tumour cells resistant to a range of chemotherapeutic drugs. We have examined five cultured human oesophageal squamous carcinoma cell lines and one epidermoid carcinoma cell line for the expression of P-glycoprotein using a radioimmuno assay developed for the purpose. The radioimmuno assays identified significantly different (P = 0.05) levels of P-glycoprotein among the five oesophageal carcinoma cell lines. Treating the lines with epidermal growth factor caused them to increase their P-glycoprotein expression exponentially. Exposing the lines to vinblastine identified four of the five oesophageal carcinoma cell lines as having some resistance to the drug. The SNO cell line expressed a drug-sensitive phenotype with cell death occurring at low drug concentrations. This drug sensitivity was reversed by prior treatment with epidermal growth factor. The high levels of P-glycoprotein generated by the cell lines may be linked to the poor success rate reported for chemotherapeutic treatment of oesophageal carcinoma.
- Subjects
ESOPHAGEAL cancer; CELL lines; TUMORS; P-glycoprotein
- Publication
South African Journal of Science, 2001, Vol 97, Issue 7/8, p329
- ISSN
0038-2353
- Publication type
Article