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- Title
Overexpression of Three Duplicated BnPCS Genes Enhanced Cd Accumulation and Translocation in Arabidopsis thaliana Mutant cad1-3.
- Authors
Bai, Jiuyuan; Wang, Xin; Wang, Rui; Wang, Jing; Le, Sixiu; Zhao, Yun
- Abstract
Phytochelatins are widely known to chelate heavy metal in vacuole and decrease plant damage. Phytochelatin synthase gene (PCS), which is involved in phytochelatins synthesis, is commonly designated as a key gene for phytoremediation. In our study, we cloned three duplicated BnPCS genes from Brassica napus and transformed them into Arabidopsis thaliana AtPCS1 mutant cad1-3, respectively. Three transgene lines and cad1-3 were subjected to a cascade of concentrations of cadmium (Cd) treatment. Evaluation of morphological and physiological measurement results show that transgene lines possess higher Cd tolerance and resistance than A. thaliana mutant cad1-3. The analysis of PCs and Cd contents in root and shoot collectively indicated that transgenic plants promoted Cd accumulation and translocation. In conclusion, all the three BnPCS transgene lines enhanced Cd tolerance, accumulation and translocation, which could provide gene resources for phytoremediation.
- Subjects
PLANT genes; ARABIDOPSIS thaliana; PHYTOCHELATINS; HEAVY metals; PLANT vacuoles; SYNTHASE genetics; PHYTOREMEDIATION
- Publication
Bulletin of Environmental Contamination & Toxicology, 2019, Vol 102, Issue 1, p146
- ISSN
0007-4861
- Publication type
Article
- DOI
10.1007/s00128-018-2487-1