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- Title
Characterization of two genes encoding leucine-rich repeat-containing proteins in grass carpCtenopharyngodon idellus.
- Authors
Chang, M.; Nie, P.; Xie, H.; Sun, B.; Gao, Q.
- Abstract
The cDNAs and genes of two different types of leucine-rich repeat-containing proteins from grass carp (Ctenopharyngodon idellus) were cloned. Homology search revealed that the two genes, designated asGC-GARPandGC-LRG, have 37% and 32% deduced amino-acid sequence similarities with human glycoprotein A repetitions predominant precursor (GARP) and leucine-rich a2-glycoprotein (LRG), respectively. The cDNAs ofGC-GARPandGC-LRGencoded 664 and 339 amino acid residues, respectively.GC-GARPandGC-LRGcontain many distinct structural and/or functional motifs of the leucine-rich repeat (LRR) subfamily, such as multiple conserved 11-residue segments with the consensus sequence LxxLxLxxN/CxL (x can be any amino acid). The genesGC-GARPandGC-LRGconsist of two exons, with 4,782 bp and 2,119 bp in total length, respectively. The first exon of each gene contains a small 5'-untranslated region and partial open reading frame. The putative promoter region ofGC-GARPwas found to contain transcription factor binding sites for GATA-1, IRF4, Oct-1, IRF-7, IRF-1, AP1, GATA-box and NFAT, and the promoter region ofGC-LRGfor MYC-MAX, MEIS1, ISRE, IK3, HOXA9 and C/EBP alpha. Phylogenetic analysis showed that GC-GARP and mammalian GARPs were clustered into one branch, while GC-LRG and mammalian LRGs were in another branch. TheGC-GARPgene was only detected in head kidney, andGC-LRGin the liver, spleen and heart in the copepod (Sinergasilus major)-infected grass carp, indicating the induction of gene expression by the parasite infection. The results obtained in the present study provide insight into the structure of fishLRRgenes, and further study should be carried out to understand the importance of LRR proteins in host-pathogen interactions.
- Subjects
CTENOPHARYNGODON idella; FISHES; GENES; PROTEINS; DNA; LEUCINE; HOMOLOGY (Biology)
- Publication
Immunogenetics, 2005, Vol 56, Issue 10, p710
- ISSN
0093-7711
- Publication type
Article
- DOI
10.1007/s00251-004-0737-3