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- Title
Mixed-type gastric cancer and its association with high-frequency CpG island hypermethylation.
- Authors
Seog-Yun Park; Myeong Cherl Kook; Young Woo Kim; Nam-Yun Cho; Tae-You Kim; Gyeong Hoon Kang; Park, Seog-Yun; Kook, Myeong Cherl; Kim, Young Woo; Cho, Nam-Yun; Kim, Tae-You; Kang, Gyeong Hoon
- Abstract
Gastric carcinoma (GC) is one of the human cancers in which promoter CpG island hypermethylation is frequently found. We used the MethyLight assay to evaluate the methylation status of 16 CpG island loci that are hypermethylated in GC. We analyzed the relationship between CpG island hypermethylation of these 16 genes and the clinicopathological features in 191 advanced GCs. A significant difference was observed between the number of methylated genes in Epstein-Barr virus (EBV)-negative and microsatellite instability (MSI)-negative GCs of different histological types (Lauren classification; P < 0.01). We found that mixed-type (MT) carcinomas, which have both diffuse-type (DT) and intestinal-type (IT) components, had more methylated genes (10.6) than either DT carcinomas (7.6 methylated genes) or IT carcinomas (6.7 methylated genes) (P < 0.001). This trend was also observed when EBV-positive or MSI-positive GCs were excluded from the analysis (9.2, 6.9, and 4.8; P < 0.001). When the IT and DT components were dissected from MT carcinomas and the methylation of these 16 genes was evaluated, both components had a number of methylated genes similar to MT carcinomas, (10.2 and 9.7, respectively), which was significantly higher than was found in IT and DT carcinomas (P < 0.05). These findings indicate that MT carcinoma is distinct from IT and DT carcinomas in its enhanced CpG island hypermethylation status and implicate the enhanced promoter CpG island hypermethylation in the histogenesis of MT carcinoma.
- Subjects
STOMACH cancer; METHYLATION; DNA; MICROSATELLITE repeats; CHROMOSOMES
- Publication
Virchows Archiv: European Journal of Pathology, 2010, Vol 456, Issue 6, p625
- ISSN
0945-6317
- Publication type
journal article
- DOI
10.1007/s00428-010-0916-6