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- Title
Analysis of glomerular VEGF mRNA and protein expression in murine mesangioproliferative glomerulonephritis.
- Authors
Christian Haas; Valentina Câmpean; Alexander Kuhlmann; Arno Dimmler; Udo Reulbach; Christian Forster; Thomas Aigner; Till Acker; Karl Plate; Kerstin Amann
- Abstract
Abstract??Capillary repair is crucial in the healing of glomerulonephritis (GN). The vascular endothelial growth factor (VEGF) has pro-angiogenic properties and plays an important role in glomerular capillary regeneration. Habu Snake Venom (HSV) GN, a murine model for mesangioproliferative GN, was induced in uninephrectomized C57/BL6 mice. Glomerular damage and capillary repair were assessed using morphometry, stereology, and confocal laser scanning microscopy. Mesangiolytic glomeruli were microdissected (days?1,3,7,14) using laser capture microdissection technique. VEGF mRNA expression was analyzed by real-time polymerase chain reaction and compared to intact glomeruli of healthy controls. SpatiotemporalVEGFgene and protein expression was determined using nonradioactive in situ hybridization and immunohistochemistry. On day?1, diseased animals developed focal mesangiolysis paralleled by a significant decrease in length density of glomerular capillaries that gradually returned to baseline levels thereafter, indicating capillary growth in response to initial injury. Glomerular VEGF mRNA expression increased on day?3 and returned back to baseline and beyond at day?14 when the glomerular recovery process was completed. Similarly, glomerular VEGF protein expression tended to be higher on day?3. The present study documents temporarily increased glomerularVEGFgene and protein expression during the healing of HSV GN, suggesting a potential role of VEGF in the repair of mesangiolytic glomerular damage.
- Subjects
GLOMERULONEPHRITIS; VASCULAR endothelial growth factors; IMMUNOHISTOCHEMISTRY; MICRODISSECTION
- Publication
Virchows Archiv: European Journal of Pathology, 2007, Vol 450, Issue 1, p81
- ISSN
0945-6317
- Publication type
Article
- DOI
10.1007/s00428-006-0340-0