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- Title
Identification of MHC I class genes in two Platyrrhini species.
- Authors
Cao, Yu‐Hua; Fan, Jun‐Wen; Li, Ai‐Xue; Liu, Hui‐Fang; Li, Lian‐Rui; Zhang, Cheng‐Lin; Zeng, Lin; Sun, Zhao‐Zeng
- Abstract
The major histocompatibility complex is a diverse gene family that plays a crucial role in the adaptive immune system. In humans, the MHC class I genes consist of the classical loci of HLA-A, -B, and -C, and the nonclassical loci HLA-E, -F, and -G. In Platyrrhini species, few MHC class I genes have been described so far and were classified as MHC-E, MHC-F, and MHC-G, with MHC-G possibly representing a classical MHC class I locus while there were arguments about the existence of the MHC-B locus in Platyrrhini. In this study, MHC class I genes were identified in eight common marmosets (Callithrix jacchus) and two brown-headed spider monkeys (Ateles fusciceps). For common marmosets, 401 cDNA sequences were sequenced and 18 alleles were detected, including 14 Caja-G alleles and 4 Caja-B alleles. Five to eleven Caja-G alleles and one to three Caja-B alleles were detected in each animal. For brown-headed spider monkeys, 102 cDNA sequences were analyzed, and 9 new alleles were identified, including 5 Atfu-G and 4 Atfu-B alleles. Two or three Atfu-G and two Atfu-B alleles were obtained for each of animal. In phylogenetic analyses, the MHC-G and -B alleles from the two species and other Platyrrhini species show locus-specific clusters with bootstrap values of 86% and 50%. The results of pairwise sequence comparisons and an excess of non-synonymous nucleotide substitutions in the PBR region are consistent with the suggestion that Caja-G and Atfu-G may be classical MHC class I loci in the Platyrrhini species... But it appears that MHC-B locus of the two Platyrrhini species shares features with both classical and nonclasical MHC class I loci. Our results are an important addition to the limited MHC immunogenetic information available for the Platyrrhini species. Am. J. Primatol. 77:527-534, 2015. © 2015 Wiley Periodicals, Inc.
- Subjects
CALLITHRIX; MARMOSETS; ANTISENSE DNA; SPIDER monkeys; NEW World monkeys; MAJOR histocompatibility complex
- Publication
American Journal of Primatology, 2015, Vol 77, Issue 5, p527
- ISSN
0275-2565
- Publication type
Article
- DOI
10.1002/ajp.22372