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- Title
Phylogeny of the Brachytheciaceae (Bryophyta) based on morphology and sequence level data.
- Authors
Huttunen, Sanna; Ignatov, Michael S.
- Abstract
Brachytheciaceae is often considered a taxonomicallydifficult group of mosses. For example, morphological variation has led to difficulty in generic delimitation. We used DNA sequence data (chloroplastpsbT-H andtrnL-F and nuclear ITS2) together with morphology (63 characters) to examine the relationships within this family. The combined unaligned length of the DNA sequences used in the phylogenetic analyses varied between 1277 and 1343 bp. For phylogeny reconstruction we performed direct optimization, as implemented in POY. Analyses were performed with three different gap costs and the morphological data partition was weighted both: (1) equal to gap cost, and (2) with a weight of one. The utility of sensitivity analysis has recently been cast into doubt; hence in this study it was performed only to explore the effects of weighting on homology statements and topologies and to enable more detailed comparisons between earlier studies utilizing the direct optimization method. The wide sequence length variation of non-coding ITS2 sequences resulted in character optimizations (i.e.,“alignments”) of very different lengths when various gap costs were applied. Despite this variation, the topologies of equally parsimonious trees remained fairly stable. The inclusion of several outgroups, instead of only one, was observed to increase the congruence between data sets and to slightly increase the resolution. An inversion event in the 9 bp loop region in the chloroplastpsbT-N spacer in mosses has been postulated to include only uninformative variation, thus possibly negatively impacting the phylogeny reconstruction. Despite this inversion, its variation within Brachytheciaceae was clearly congruent with information from other sources, but inclusion of these 9 bp in the analysis had only a minor effect on the phylogenetic results. In the most parsimonious topology, which was obtained with equal weighting of all data, Meteoriaceae and Brachytheciaceae were resolved as monophyletic sister groups, which had recently been suggested based on a few shared morphological characters. Our study revealed some new generic relationships within the Brachytheciaceae, which are discussed in light of the morphological characters traditionally used for generic delimitation.
- Subjects
MOSSES; NUCLEOTIDE sequence; BRACHYTHECIACEAE; BRYOPHYTES; PHYLOGENY; BIOLOGY
- Publication
Cladistics, 2004, Vol 20, Issue 2, p151
- ISSN
0748-3007
- Publication type
Article
- DOI
10.1111/j.1096-0031.2004.00022.x