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- Title
石竹叶斑病病原鉴定.
- Authors
李番; 任毓忠; 焦瑞莲; 孙璘; 李国英
- Abstract
[Objective] The aim of this project is to clarify the pathogen of Dianthus chinensis leaf spot in Shihezi area and control the disease. [Methods] Using conventional organic law, 8 representative strains were selected and their pathogenicity was determined by mycelium patch and spray method. The morphological and rDNA-ITS regions, histone 3 and β- tubulin sequences were compared and analyzed to establish a multi-gene combined phylogenetic tree to determine the classification status of pathogens. [Results] Morphologically, the conidium was similar to Alternaria nobilis. The sequence analysis of rDNA-ITS and β-tubulin of 8 isolates were more than 99.0% homologous to the reported phylogeny of Alternaria nobilis in GenBank. [Conclusion] Combined with the morphological characteristics and molecular biological results, the pathogen causing the Dianthus chinensis leaf spot is identified as Alternaria. nobilis in Shihezi area.
- Publication
Xinjiang Agricultural Sciences, 2021, Vol 58, Issue 4, p712
- ISSN
1001-4330
- Publication type
Article
- DOI
10.6048/j.issn.1001-4330.2021.04.014