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- Title
COMPARISON OF THREE METHODS IN THE DIAGNOSIS OF CRYPTOSPORIDIUM PARVUM PARASITE IN DIARRHEA SAMPLES IN BABYLON PROVINCE, IRAQ.
- Authors
Obayes, Israa Khudhair; Khadim, Samah Sajad; Sallal, Hiba Jabur; Hamza, Zaman S.
- Abstract
The present study aimed at using more than one method for the diagnosis of C. parvum parasites in feces samples taken from patients with diarrhea in Babylon province. Fecal samples initially examined by two methods, modified acid-fast stain and chromatographic immunoassay. Positive samples in the chromatographic immunoassay method examined by molecular method using two specific genes of this parasite (dihydrofolate reductase gene DHFR and 70-Kilodalton Heat Shock Protein Gene HSP70). The results of the present study showed that the percentage of C. parvum infection were 4.95% and 3.58% in the acid-fast stain and chromatographic immunoassay methods, respectively. Statistical analysis showed that there is no significant difference between the percentages of infection in the two methods. The results of the DNA extraction of positive samples in chromatographic immunoassay method and the use of two genes for this parasite (HPS70 and CH-DHFR) showed that only nine samples gave a positive result out of 13 with percentage of infection equal 2.47%. We conclude from the results of the current study that the C. parvum one of the parasitic causes of diarrhea in the province of Babylon, despite the low percentage of infection and the possibility of using more than one method to diagnose.
- Subjects
IRAQ; BABYLON (Extinct city); CRYPTOSPORIDIUM; UREAPLASMA; HSP70 heat-shock proteins; CRYPTOSPORIDIUM parvum; TETRAHYDROFOLATE dehydrogenase; DIAGNOSIS methods; DIARRHEA
- Publication
Biochemical & Cellular Archives, 2022, Vol 22, Issue 1, p761
- ISSN
0972-5075
- Publication type
Article