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- Title
桃4CL基因家族鉴定及其在果实色泽发育和采后贮藏冷害中的表达分析.
- Authors
孔凡旺; 张志刚; 李伟; 陈玉峰; 王长江; 郑亚琴; 徐蒙
- Abstract
4CL (4-coumarate: CoA ligase) plays an important role in the metabolism of phenylalanine compounds such as flavonoids and lignin. In this study, the peach 4CL gene family members were systematically identified based on the peach genome database, and the structure and possible functions of the peach 4CLs were analyzed using the genetic family evolutionary analysis, combing transcriptome data and qRT-PCR techniques to analyze their functions in flavonoids synthesis at the fruit development stage and chilling-induced lignification regulation at postharvest. The results showed that 21 members of the 4CL gene family, namely Pp4CL1-Pp4CL21, were identified on 8 chromosomes based on the peach genome database. Phylogenetic analysis showed that Pp4CL1 and Pp4CL2 belong to the first subfamily, mainly involved in the synthesis of lignin, and Pp4CL3-Pp4CL21 belongs to the second subfamily, mainly involved in the synthesis of flavonoids. The cis-element analysis of the 4CL promoters found that it contains a large number of abiotic stress and MeJA-responsive and other hormone response elements. The gene expression of different peach varieties at the development stage showed that Pp4CL8 and Pp4CL13 increased generally, and higher expression was observed in blood-flesh varieties than in yellow-and milk-white-flesh varieties, suggesting that these genes may participate in the fruit flavonoids synthesis. The gene expression of peach fruit during post-harvest cold storage showed that Pp4CL2 may play a role in the chilling-induced lignification of fruit flesh after harvest. After low temperature conditioning (LTC) treatment, Pp4CL8, Pp4CL10 and Pp4CL14 may reduce the chilling injury of peach fruit during post-harvest cold storage by improving flavonoids content. This study provides a theoretical basis for the coloration of peach fruit and its postharvest preservation.
- Publication
Acta Agriculturae Zhejiangensis, 2023, Vol 35, Issue 11, p2600
- ISSN
1004-1524
- Publication type
Article
- DOI
10.3969/j.issn.1004-1524.20221604