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- Title
Surface-associated antigen induces permeabilization of primary mouse Bcells and lysosome exocytosis facilitating antigen uptake and presentation to T-cells.
- Authors
Maeda, Fernando Y.; van Haaren, Jurriaan J. H.; Langley, David B.; Christ, Daniel; Andrews, Norma W.; Wenxia Song
- Abstract
B-cell receptor (BCR)-mediated antigen internalization and presentation are essential for humoral memory immune responses. Antigen encountered by B-cells is often tightly associated with the surface of pathogens and/or antigen-presenting cells. Internalization of such antigens requires myosin- mediated traction forces and extracellular release of lysosomal enzymes, but the mechanism triggering lysosomal exocytosis is unknown. Here, we show that BCR-mediated recognition of antigen tethered to beads, to planar lipid- bilayers or expressed on cell surfaces causes localized plasma membrane (PM) permeabilization, a process that requires BCR signaling and non-muscle myosin II activity. B-cell permeabilization triggers PM repair responses involving lysosomal exocytosis, and B- cells permeabilized by surface-associated antigen internalize more antigen than cells that remain intact. Higher affinity antigens cause more B- cell permeabilization and lysosomal exocytosis and are more efficiently presented to T-cells. Thus, PM permeabilization by surface-associated antigen triggers a lysosome- mediated B-cell resealing response, providing the extracellular hydrolases that facilitate antigen internalization and presentation.
- Subjects
ANTIGEN presentation; EXOCYTOSIS; CELL membranes; T cells; ANTIGENS; LYSOSOMES; IMMUNE recognition
- Publication
eLife, 2021, p1
- ISSN
2050-084X
- Publication type
Article
- DOI
10.7554/eLife.66984