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- Title
Overexpression, purification, crystallization and preliminary X-ray crystallographic analysis of SF173 from Shigella flexneri.
- Authors
Kim, Ha-Neul; An, Jeong-Gi; Lee, Yoo-Sup; Seok, Seung-Hyeon; Yoo, Hee-Seop; Seo, Min-Duk
- Abstract
Shigella flexneri is a Gram-negative, anaerobic bacterium in the genus Shigella that can cause diarrhoea in humans. SF173, a hypothetical protein from S. flexneri 5a strain M90T, has been cloned, overexpressed, purified and crystallized as a part of laboratory-scale structural genomics project. The SF173 protein was crystallized using the sitting-drop vapour-diffusion method in the presence of 0.8 M succinic acid pH 7.0 at 293 K. Preliminary X-ray diffraction analysis revealed that the crystal diffracted to 1.47 Å resolution and belonged to space group I432, with unit-cell parameters a = b = c = 110.245 Å.
- Subjects
SHIGELLA flexneri; CRYSTALLIZATION; MEMBRANE proteins; ANTIBIOTICS; X-ray diffraction
- Publication
Acta Crystallographica: Section F, Structural Biology Communications, 2015, Vol 71, Issue 1, p54
- ISSN
2053-230X
- Publication type
Article
- DOI
10.1107/S2053230X14025941