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- Title
Hint1 gene deficiency enhances the supraspinal nociceptive sensitivity in mice.
- Authors
Liu, Fei; Ma, Jing; Liu, Peng; Chu, Zheng; Lei, Gang; Jia, Xiao‐di; Wang, Jia‐bei; Dang, Yong‐hui
- Abstract
Introduction Previous studies have indicated a possible role of histidine triad nucleotide-binding protein 1 ( HINT1) on sustaining the regulatory crosstalk of N-methyl-D-aspartate acid glutamate receptors ( NMDARs) and G-protein-coupled receptors ( GPCRs) such as the μ-opioid receptor ( MOR). Both receptors are present in the midbrain periaqueductal gray neurons, an area that plays a central role in the supraspinal antinociceptive process. Methods In the present study, a battery of pain-related behavioral experiments was applied to Hint1 knockout, heterozygous and wild-type mice. Both the male and female mice were investigated to assess the differences between genders. Results Hint1−/− mice presented significant shorter latency at 50°C in both male and female in hot plate test while no significant difference was found in tail filck test. In Von Frey hairs test Hint1−/− mice were more sensitive than Hint1+/+ mice, presenting a lower withdrawal threshold and enhanced relative frequency of paw withdrawal. The average flinches and licking time of Hint1−/− mice were more than that of Hint1+/+ mice in formalin test. Conclusion The absence of Hint1 gene-enhanced supraspinal nociceptive sensitivity in mice, including thermal, mechanical and inflammatory hyperalgesia. Meanwhile, there was no certain evidence indicating the haploinsufficiency and gender differences of Hint1 gene in pain-related behaviors.
- Subjects
GENDER differences (Psychology); HISTIDINE kinases; NOCICEPTIVE pain; CARRIER proteins; METHYL aspartate receptors; OPIOID receptors; HYPERALGESIA; LABORATORY mice; GENETICS
- Publication
Brain & Behavior, 2016, Vol 6, Issue 8, pn/a
- ISSN
2162-3279
- Publication type
Article
- DOI
10.1002/brb3.496