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- Title
Quantitative measurement of hydrophilicity/ hydrophobicity of the plasma-polymerized naphthalene film (Super Support Film) and other support films and grids in electron microscopy.
- Authors
Masashi Yamaguchi; Toshihiro Aoyama; Norihiro Yamada; Hiroji Chibana
- Abstract
Hydrophilicity/hydrophobicity of the surfaces of plasma-polymerized naphthalene film (Super Support Film, Nisshin EM Co. Ltd., Tokyo), carbon and formvar support films, and copper and nickel grids were quantitatively estimated by contact angles measured from diameters and heights of water droplets placed on various specimens. With treatment of glow discharge, the surfaces of plasma-polymerized naphthalene and carbon support films became fully hydrophilic in 20 s. They remained hydrophilic for 6 h. The surfaces of copper and nickel grids became fully hydrophilic with 60 s of glow discharge treatment. They remained hydrophilic for only 1 h. This information is useful for negative staining, ultrathin sectioning and rapid freezing of biological specimens using the sandwich method.
- Subjects
PLASMA polymerization; NAPHTHALENE; ELECTRON microscopy; COPPER; NICKEL
- Publication
Microscopy, 2016, Vol 65, Issue 5, p444
- ISSN
2050-5698
- Publication type
Article
- DOI
10.1093/jmicro/dfw031