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- Title
Prevalence and distribution of Grapevine leafroll-associated virus 7 in China detected by an improved reverse transcription polymerase chain reaction assay.
- Authors
Lyu, M. D.; Li, X. M.; Guo, R.; Li, M. J.; Liu, X. M.; Wang, Q.; Cheng, Y. Q.
- Abstract
To determine prevalence and distribution of Grapevine leafroll-associated virus 7 (GLRaV-7) in China, a total of 213 grapevine (Vitis spp.) samples (92 popular cultivars) from 13 Chinese provinces and regions were tested for the presence of GLRaV-7 by reverse transcription polymerase chain reaction (RT-PCR) assays and sequencing. GLRaV-7 was found in 40·4% of the samples, and shown to be widely distributed in major grapevine growing areas in China. GLRaV-7 was found in more than 50 grapevine cultivars. Some popular grape cultivars showed a high incidence of GLRaV-7 infection, such as Manicure Finger (100%), Cabernet Sauvignon (83·3%), Merlot (60%), Fujimineri (60%) and Red Globe (50%). The genetic variability of GLRaV-7 isolates was characterized based on partial nucleotide sequences (nucleotides 945-1329 and 97-598 of the ORF) of heat shock protein 70 homologue (HSP70h) and 61-kDa protein (p61) (nucleotides 1073-1572 of the ORF) genes. The overall mean values of nucleotide diversity were low (ranging from 0·009 to 0·066), and phylogenetic analysis based on p61 showed that the GLRaV-7 isolates segregated into three phylogenetic clusters. The results also showed that two previously described primer pairs failed to amplify a wide range of GLRaV-7 isolates present in China. However, a new primer pair reported here, based on the p61 gene and its associated downstream intergenic region, detected more GLRaV-7 Chinese isolates. This is the first report on the prevalence and distribution of GLRaV-7 in China, and also provides an improved RT-PCR assay for detecting the virus.
- Subjects
CHINA; DETECTION of phytopathogenic microorganisms; GRAPEVINE leafroll virus; REVERSE transcriptase polymerase chain reaction; GRAPE varieties; NUCLEOTIDE sequence
- Publication
Plant Pathology, 2014, Vol 63, Issue 5, p1168
- ISSN
0032-0862
- Publication type
Article
- DOI
10.1111/ppa.12195