We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Distinct roles for U‐type proteins in iron–sulfur cluster biosynthesis revealed by genetic analysis of the <italic>Bacillus subtilis sufCDSUB</italic> operon.
- Authors
Yokoyama, Nao; Nonaka, Chihiro; Ohashi, Yukari; Shioda, Masaharu; Terahata, Takuya; Chen, Wen; Sakamoto, Kotomi; Maruyama, Chihiro; Saito, Takuya; Yuda, Eiki; Tanaka, Naoyuki; Fujishiro, Takashi; Kuzuyama, Tomohisa; Asai, Kei; Takahashi, Yasuhiro
- Abstract
Summary: The biosynthesis of iron–sulfur (Fe–S) clusters in <italic>Bacillus subtilis</italic> is mediated by the SUF‐like system composed of the <italic>sufCDSUB</italic> gene products. This system is unique in that it is a chimeric machinery comprising homologues of <italic>E. coli</italic> SUF components (SufS, SufB, SufC and SufD) and an ISC component (IscU). <italic>B. subtilis</italic> SufS cysteine desulfurase transfers persulfide sulfur to SufU (the IscU homologue); however, it has remained controversial whether SufU serves as a scaffold for Fe–S cluster assembly, like IscU, or acts as a sulfur shuttle protein, like <italic>E. coli</italic> SufE. Here we report that reengineering of the isoprenoid biosynthetic pathway in <italic>B. subtilis</italic> can offset the indispensability of the <italic>sufCDSUB</italic> operon, allowing the resultant Δ<italic>suf</italic> mutants to grow without detectable Fe–S proteins. Heterologous bidirectional complementation studies using <italic>B. subtilis</italic> and <italic>E. coli</italic> mutants showed that <italic>B. subtilis</italic> SufSU is interchangeable with <italic>E. coli</italic> SufSE but not with IscSU. In addition, functional similarity in SufB, SufC and SufD was observed between <italic>B. subtilis</italic> and <italic>E. coli</italic>. Our findings thus indicate that <italic>B. subtilis</italic> SufU is the protein that transfers sulfur from SufS to SufB, and that the SufBCD complex is the site of Fe–S cluster assembly.
- Subjects
PROTEIN analysis; BIOSYNTHESIS; IRON clusters; SULFUR analysis; BACILLUS subtilis genetics; ESCHERICHIA coli; ISOPENTENOIDS
- Publication
Molecular Microbiology, 2018, Vol 107, Issue 6, p688
- ISSN
0950-382X
- Publication type
Article
- DOI
10.1111/mmi.13907