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- Title
Genome-Wide Identification and Expression Pattern of Sugar Transporter Genes in the Brown Planthopper, Nilaparvata lugens (Stål).
- Authors
Shangguan, Xinxin; Yang, Xiaoyu; Wang, Siyin; Geng, Lijie; Wang, Lina; Zhao, Mengfan; Cao, Haohao; Zhang, Yi; Li, Xiaoli; Yang, Mingsheng; Xu, Kedong; Zheng, Xiaohong
- Abstract
Simple Summary: The brown planthopper, Nilaparvata lugens, is a destructive insect pest that poses a serious threat to rice production. Because the brown planthopper is a phloem-sap-feeding insect and relies on sugars as their main source of carbon, sugar transporters are particularly important. We identified 34 sugar transporters in N. lugens (NlSTs) and inferred their possible function based on their expression patterns. We identified a number of NlST genes that are expressed at higher levels in the gut than in other tissues, such as NlST2, 3, 4, 7, 20, 27, 28, and 31. This study provides a basis for further exploring the function of NlST genes in brown planthopper. Sugar transporters play important roles in controlling carbohydrate transport and are responsible for mediating the movement of sugars into cells in numerous organisms. In insects, sugar transporters not only play a role in sugar transport but may also act as receptors for virus entry and the accumulation of plant defense compounds. The brown planthopper, Nilaparvata lugens, inflicts damage on rice plants by feeding on their phloem sap, which is rich in sugars. In the present study, we identified 34 sugar transporters in N. lugens, which were classified into three subfamilies based on phylogenetic analysis. The motif numbers varied from seven to eleven, and motifs 2, 3, and 4 were identified in the functional domains of all 34 NlST proteins. Chromosome 1 was found to possess the highest number of NlST genes, harboring 15. The gut, salivary glands, fat body, and ovary were the different tissues enriched with NlST gene expression. The expression levels of NlST2, 3, 4, 7, 20, 27, 28, and 31 were higher in the gut than in the other tissues. When expressed in a Saccharomyces cerevisiae hexose transporter deletion mutant (strain EBY.VW4000), only ApST4 (previously characterized) and NlST4, 28, and 31 were found to transport glucose and fructose, resulting in functional rescue of the yeast mutant. These results provide valuable data for further studies on sugar transporters in N. lugens and lay a foundation for finding potential targets to control N. lugens.
- Subjects
INSECT pests; NILAPARVATA lugens; GENE expression; PLANT defenses; SALIVARY glands
- Publication
Insects (2075-4450), 2024, Vol 15, Issue 7, p509
- ISSN
2075-4450
- Publication type
Article
- DOI
10.3390/insects15070509