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- Title
Tagging the Signatures of Domestication in Common Bean (Phaseolus vulgaris) by Means of Pooled DNA Samples.
- Authors
Roberto Papa; Elisa Bellucci; Monica Rossi; Stefano Leonardi; Domenico Rau; Paul Gepts; Laura Nanni; Giovanna Attene
- Abstract
Background and Aims The main aim of this study was to use an amplified fragment length polymorphism (AFLP)-based, large-scale screening of the whole genome of Phaseolus vulgaris to determine the effects of selection on the structure of the genetic diversity in wild and domesticated populations. Methods Using pooled DNA samples, seven each of wild and domesticated populations of P. vulgaris were studied using 2506 AFLP markers (on average, one every 250 kb). About 10 % of the markers were also analysed on individual genotypes and were used to infer allelic frequencies empirically from bulk data. In both data sets, tests were made to determine the departure from neutral expectation for each marker using an FST-based method. Key Results The most important outcome is that a large fraction of the genome of the common bean (16 %; P Conclusions Overall, the results indicate that in P. vulgaris a large portion of the genome appears to have been subjected to the effects of selection, probably because of linkage to the loci selected during domestication. As most of the markers that are under the effects of selection are linked to known loci related to the domestication syndrome, it is concluded that population genomics approaches are very efficient in detecting QTLs. A method based on bulk DNA samples is presented that is effective in pre-screening for a large number of markers to determine selection signatures.
- Subjects
BEAN genetics; DNA; GENETIC polymorphisms; DOMESTICATION of plants
- Publication
Annals of Botany, 2007, Vol 100, Issue 5, p1039
- ISSN
0305-7364
- Publication type
Article
- DOI
10.1093/aob/mcm151