We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Evaluation of zoonotic potency of Escherichia coli O157:H7 through arbitrarily primed PCR methods.
- Authors
Suardana, I Wayan; Widiasih, Dyah Ayu; Mahardika, I Gusti Ngurah Kade; Pinatih, Komang Januartha Putra; Daryono, Budi Setiadi
- Abstract
Objective To evaluate the zoonotic potency of Escherichia coli O157:H7 through arbitrarily primed-PCR (AP-PCR) methods as one of the DNA fingerprinting methods. Methods A total of 14 isolates consisted of 11 isolates originated from human feces with renal failure symptoms, 2 isolates originated from cattle feces, and 1 control isolate were used in this study. DNA of each isolate was extracted, and their profiles were studied by using AP-PCR method with M13 F and M13 R arbitrary primers. Results The results founded that all of 14 isolates had similarity range from 54.6% to 88.5%. Isolates KL-106(3) and KL-55(6) originated from humans showed the degree of similarity with isolates SM-25(1) and SM-7(1) originated from cattle as high as 85% and 77%, respectively. Conclusions The high degree of similarity between isolates originated from cattle and human indicated the high potency of zoonoses. The results also concluded AP-PCR method as a briefly fingerprinting method in order to trace the epidemiological of E. coli O157:H7.
- Subjects
ESCHERICHIA coli O157:H7; POLYMERASE chain reaction; DNA fingerprinting; ZOONOSES; KIDNEY failure
- Publication
Asian Pacific Journal of Tropical Biomedicine, 2015, Vol 5, Issue 11, p915
- ISSN
2221-1691
- Publication type
Article
- DOI
10.1016/j.apjtb.2015.07.023