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- Title
Linkage Mapping and Comparative Genomics of Red Drum (Sciaenops ocellatus) Using Next-Generation Sequencing.
- Authors
Hollenbeck, Christopher M.; Portnoy, David S.; Wetzel, Dana; Sherwood, Tracy A.; Samollow, Paul B.; Gold, John R.
- Abstract
Developments in next-generation sequencing allow genotyping of thousands of genetic markers across hundreds of individuals in a cost-effective manner. Because of this, it is now possible to rapidly produce dense genetic linkage maps for nonmodel species. Here, we report a dense genetic linkage map for red drum, a marine fish species of considerable economic importance in the southeastern United States and elsewhere. We used a prior microsatellite-based linkage map as a framework and incorporated 1794 haplotyped contigs derived from high-throughput, reduced representation DNA sequencing to produce a linkage map containing 1794 haplotyped restriction-site associated DNA (RAD) contigs, 437 anonymous microsatellites, and 44 expressed sequence-tag-linked microsatellites (EST-SSRs). A total of 274 candidate genes, identified from transcripts from a preliminary hydrocarbon exposure study, were localized to specific chromosomes, using a shared synteny approach. The linkage map will be a useful resource for red drum commercial and restoration aquaculture, and for better understanding and managing populations of red drum in the wild.
- Subjects
RED drum (Fish); GENE mapping; NUCLEOTIDE sequencing; FISHES
- Publication
G3: Genes | Genomes | Genetics, 2017, Vol 7, Issue 3, p843
- ISSN
2160-1836
- Publication type
Article
- DOI
10.1534/g3.116.036350