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- Title
Sxl-Dependent, tra/tra2-Independent Alternative Splicing of the Drosophila melanogaster X-Linked Gene found in neurons.
- Authors
Xia Sun; Haiwang Yang; Sturgill, David; Oliver, Brian; Rabinow, Leonard; Samson, Marie-Laure
- Abstract
Somatic sexual determination and behavior in Drosophila melanogaster are under the control of a genetic cascade initiated by Sex lethal (Sxl). In the female soma, SXL RNA-binding protein regulates the splicing of transformer (tra) transcripts into a female-specific form. The RNA-binding protein TRA and its cofactor TRA2 function in concert in females, whereas SXL, TRA, and TRA2 are thought to not function in males. To better understand sex-specific regulation of gene expression, we analyzed male and female head transcriptome datasets for expression levels and splicing, quantifying sex-biased gene expression via RNASeq and qPCR. Our data uncouple the effects of Sxl and tra/tra2 in females in the-sex-biased alternative splicing of head transcripts from the X-linked locus found in neurons (fne), encoding a pan-neuronal RNAbinding protein of the ELAV family. We show that FNE protein levels are downregulated by Sxl in female heads, also independently of tra/tra2. We argue that this regulation may have important sexually dimorphic consequences for the regulation of nervous system development or function.
- Subjects
DROSOPHILA melanogaster; GENE expression; RNA-binding proteins
- Publication
G3: Genes | Genomes | Genetics, 2015, Vol 5, Issue 12, p2865
- ISSN
2160-1836
- Publication type
Article
- DOI
10.1534/g3.115.023721