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- Title
Calcium Carbonate Mineralized Nanoparticles as an Intracellular Transporter of Cytochrome c for Cancer Therapy.
- Authors
Koo, Ahn Na; Min, Kyung Hyun; Lee, Hong Jae; Jegal, Jun Ho; Lee, Jae Won; Lee, Sang Cheon
- Abstract
A new intracellular delivery system based on an apoptotic protein-loaded calcium carbonate (CaCO3) mineralized nanoparticle (MNP) is described. Apoptosis-inducing cytochrome c (Cyt c) loaded CaCO3 MNPs (Cyt c MNPs) were prepared by block copolymer mediated in situ CaCO3 mineralization in the presence of Cyt c. The resulting Cyt c MNPs had a vaterite polymorph of CaCO3 with a mean hydrodynamic diameter of 360.5 nm and exhibited 60 % efficiency for Cyt c loading. The Cyt c MNPs were stable at physiological pH (pH 7.4) and effectively prohibited the release of Cyt c, whereas, at intracellular endosomal pH (pH 5.0), Cyt c release was facilitated. The MNPs enable the endosomal escape of Cyt c for effective localization of Cyt c in the cytosols of MCF-7 cells. Flow cytometry showed that the Cyt c MNPs effectively induced apoptosis of MCF-7 cells. These findings indicate that the CaCO3 MNPs can meet the prerequisites for delivery of cell-impermeable therapeutic proteins for cancer therapy.
- Subjects
CALCIUM carbonate; NANOPARTICLES; CANCER treatment; CYTOCHROMES; HEMOPROTEINS
- Publication
Chemistry - An Asian Journal, 2015, Vol 10, Issue 11, p2380
- ISSN
1861-4728
- Publication type
Article
- DOI
10.1002/asia.201500630