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- Title
Long-Term Impact of Suppressive Antibiotic Therapy on Intestinal Microbiota.
- Authors
Escudero-Sánchez, Rosa; Ponce-Alonso, Manuel; Barragán-Prada, Hugo; Morosini, María Isabel; Cantón, Rafael; Cobo, Javier; del Campo, Rosa
- Abstract
The aim was to describe the safety of indefinite administration of antibiotics, the so-called suppressive antibiotic therapy (SAT) and to provide insight into their impact on gut microbiota. 17 patients with SAT were recruited, providing a fecal sample. Bacterial composition was determined by 16S rDNA massive sequencing, and their viability was explored by PCR-DGGE with and without propidium monoazide. Presence of antibiotic multirresistant bacteria was explored through the culture of feces in selective media. High intra-individual variability in the genera distribution regardless of the antibiotic or antibiotic administration ingestion period, with few statistically significant differences detected by Bray-Curtis distance-based principle component analysis, permutational multivariate analysis of variance and linear discriminant analysis effect size analysis. However, the microbiota composition of patients treated with both beta-lactams and sulfonamides clustered by a heat map. Curiously, the detection of antibiotic resistant bacteria was almost anecdotic and CTX-M-15-producing E. coli were detected in two subjects. Our work demonstrates the overall clinical safety of SAT and the low rate of the selection of multidrug-resistant bacteria triggered by this therapy. We also describe the composition of intestinal microbiota under the indefinite use of antibiotics for the first time.
- Subjects
GUT microbiome; BETA lactam antibiotics; FISHER discriminant analysis; ANTIBIOTICS; PROPIDIUM monoazide; FECES
- Publication
Genes, 2021, Vol 12, Issue 1, p41
- ISSN
2073-4425
- Publication type
Article
- DOI
10.3390/genes12010041