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- Title
IN VITRO TESTING OF A POLYLACTIC POLYMER SYNTHESIZED FROM WHEY.
- Authors
DREANCĂ, ALEXANDRA; POPESCU, RADU A.; NEAGU, AMALIA; ENACRACHI, GEORGE; CIMPOEŞ, IULIA; FIŢ, NICODIM; MOLDOVAN, MARIOARA; DUMITRESCU, LAURA SILAGHI; JURJ, ANCA; BERINDAN-NEAGOE, IOANA; MARCUS, IOAN
- Abstract
The main purpose of this research is in vitro biocompatibility testing of a polylactic acid polymer (PLA) synthesized by the condensation of lactic acid separated from whey (Zonar). The experimental PLA obtained was characterized by: Scanning Electron Microscopy which pointed a homogeneous microstructure of the polymer without a separation phase and differential scanning calorimetry (DSC) analysis which indicated a melting point of 1740C of the biomaterial. The cytotoxicity test for experimentally obtained polymer was performed according to ISO 10 993 -123. Six solutions of different concentrations were prepared (30 mg/ml, 20 mg/ml, 10 mg/ml, 5 mg/ml, 2.5 mg/ml and 1.25 mg/ml) and were tested on a fibroblast culture at 48 hours using MTT cell viability and proliferation assay. At 48h the obtained polylactic polymer showed good compatibility with an IC 50 of 64.82 mg/ml. The results showed that the cytotoxicity level can be influenced by the period of time in which the extract acts on the cell culture.
- Subjects
POLYLACTIC acid; POLYMERS; WHEY; BIOCOMPATIBILITY; LACTIC acid; MICROSTRUCTURE
- Publication
Studia Universitatis Babes-Bolyai, Chemia, 2018, Vol 63, Issue 2, p93
- ISSN
1224-7154
- Publication type
Article
- DOI
10.24193/subbchem.2018.2.09