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- Title
Effect of vitrification technique and assisted hatching on rabbit embryo developmental rate.
- Authors
M. Popelkov?; Z. Turanov?; L. Koprdov?; A. Ostr?; S. Toporcerov?; A. V. Makarevich; P. Chrenek
- Abstract
SummaryThe aim of the study was to determine the efficiency of two vitrification techniques followed by two assisted hatching (AH) techniques based on post-thaw developmental capacity of precompacted rabbit embryos and their ability to leave the zona pellucida (hatching) during in vitroculture. The total cell number and embryo diameter as additional markers of embryo quality after warming were evaluated. In vivofertilized, in vitrocultured 8?12-cell rabbit embryos obtained from superovulated rabbit does were cryopreserved by two-step vitrification method using ethylene glycol (EG) as cryoprotectant or by one-step vitrification method with EG and Ficoll (EG+Ficoll). Thawed embryos were subjected to enzymatic or mechanical AH. Vitrified EG group showed significantly lower (P< 0.05) blastocyst rate (22.5%) and hatching rate (15%) than those vitrified with EG + Ficoll (63 and 63% resp.) and that of control (97 and 97% respectively). Significantly lower values of total cell number (P< 0.05) as well as embryo diameter (P< 0.01) in EG group compared with EG + Ficoll and control group were recorded. No significant difference was found in developmental potential of warmed embryos treated by either mechanical or enzymatic AH. The present study demonstrates that the EG + Ficoll vitrification protocol provides superior embryo survival rates over the EG vitrification protocol for 8?12-cell stage precompacted rabbit embryos. No positive effect of either mechanical or enzymatic AH on the post-thaw viability and quality of rabbit embryos in vitrowas observed.
- Subjects
RABBIT reproduction; EMBRYOS; DEVELOPMENTAL biology; ZONA pellucida; CRYOPRESERVATION of organs, tissues, etc.; ETHYLENE glycol; REPRODUCTIVE technology
- Publication
Zygote, 2009, Vol 17, Issue 1, p57
- ISSN
0967-1994
- Publication type
Article