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- Title
Molecular Survey of Rickettsia spp. in Sick Dogs in Italy.
- Authors
Solano-Gallego, L.; Trotta, M.; Caldin, M.; Furlanello, T.
- Abstract
The aim of this retrospective study was to investigate the prevalence of Rickettsia spp. DNA in the blood of sick dogs from Italy. Canine blood samples ( n = 650) submitted for molecular testing of Rickettsia spp. to a diagnostic laboratory from February 2003 to March 2006 were studied. The Rickettsia spp. DNA detection was performed by Light Cycler real-time PCR using hybridization probes separately conducted with specific primers and probes. The total percentage of Rickettsia spp.-positive dog samples was 1.5% (10 out of 650). The percentage of Rickettsia spp.-positive dog samples submitted from north, central and southern Italy were 0.4% (1/248), 1.4% (3/219) and 3.3% (6/183), respectively. Five out of 138 dogs (3.6%) from Sicily were positive on Rickettsia PCR testing. A statistical difference was found between the percentages of positive samples from the Yorkshire terrier group (10.7%) compared with the mixed breed group (0.7%). No statistical differences were found between seasonal period, region and gender. Based on molecular data, there is infrequent rickettsiemia in dogs.
- Subjects
ITALY; RICKETTSIAL diseases in animals; DOG diseases; DISEASE prevalence; RICKETTSIA
- Publication
Zoonoses & Public Health, 2008, Vol 55, Issue 8-10, p521
- ISSN
1863-1959
- Publication type
Article
- DOI
10.1111/j.1863-2378.2008.01149.x