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- Title
Whole-Cell Biocatalysts for Stereoselective C−H Amination Reactions.
- Authors
Both, Peter; Busch, Hanna; Kelly, Paul P.; Mutti, Francesco G.; Turner, Nicholas J.; Flitsch, Sabine L.
- Abstract
Enantiomerically pure chiral amines are ubiquitous chemical building blocks in bioactive pharmaceutical products and their synthesis from simple starting materials is of great interest. One of the most attractive strategies is the stereoselective installation of a chiral amine through C−H amination, which is a challenging chemical transformation. Herein we report the application of a multienzyme cascade, generated in a single bacterial whole-cell system, which is able to catalyze stereoselective benzylic aminations with ee values of 97.5 %. The cascade uses four heterologously expressed recombinant enzymes with cofactors provided by the host cell and isopropyl amine added as the amine donor. The cascade presents the first example of the successful de novo design of a single whole-cell biocatalyst for formal stereoselective C−H amination.
- Subjects
ENZYMES; STEREOSELECTIVE reactions; AMINES; ORGANIC compounds; AMINATION kinetics; AMINATION
- Publication
Angewandte Chemie International Edition, 2016, Vol 55, Issue 4, p1511
- ISSN
1433-7851
- Publication type
Article
- DOI
10.1002/anie.201510028