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- Title
tRNA Shifts the G-quadruplex-Hairpin Conformational Equilibrium in RNA towards the Hairpin Conformer.
- Authors
Rode, Ambadas B.; Endoh, Tamaki; Sugimoto, Naoki
- Abstract
Non-coding RNAs play important roles in cellular homeostasis and are involved in many human diseases including cancer. Intermolecular RNA-RNA interactions are the basis for the diverse functions of many non-coding RNAs. Herein, we show how the presence of tRNA influences the equilibrium between hairpin and G-quadruplex conformations in the 5′ untranslated regions of oncogenes and model sequences. Kinetic and equilibrium analyses of the hairpin to G-quadruplex conformational transition of purified RNA as well as during co-transcriptional folding indicate that tRNA significantly shifts the equilibrium toward the hairpin conformer. The enhancement of relative translation efficiency in a reporter gene assay is shown to be due to the tRNA-mediated shift in hairpin-G-quadruplex equilibrium of oncogenic mRNAs. Our findings suggest that tRNA is a possible therapeutic target in diseases in which RNA conformational equilibria is dysregulated.
- Subjects
RNA; HOMEOSTASIS; RNA-protein interactions; RNA-RNA interactions; CANCER cells
- Publication
Angewandte Chemie International Edition, 2016, Vol 55, Issue 46, p14315
- ISSN
1433-7851
- Publication type
Article
- DOI
10.1002/anie.201605431