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- Title
Multiplex polymerase chain reaction assay for the detection of minute virus of mice and mouse parvovirus infections in laboratory mice.
- Authors
Wang, K. W.; Chueh, L. L.; Wang, M. H.; Huang, Y. T.; Fang, B. H.; Chang, C. Y.; Fang, M. C.; Chou, J. Y.; Hsieh, S. C.; Wan, C. H.
- Abstract
Mouse parvoviruses are among the most prevalent infectious pathogens in contemporary mouse colonies. To improve the efficiency of routine screening for mouse parvovirus infections, a multiplex polymerase chain reaction (PCR) assay targeting the VP gene was developed. The assay detected minute virus of mice (MVM), mouse parvovirus (MPV) and a mouse housekeeping gene (α-actin) and was able to specifically detect MVM and MPV at levels as low as 50 copies. Co-infection with the two viruses with up to 200-fold differences in viral concentrations can easily be detected. The multiplex PCR assay developed here could be a useful tool for monitoring mouse health and the viral contamination of biological materials.
- Subjects
POLYMERASE chain reaction; BIOLOGICAL assay; PARVOVIRUS diseases; MEDICAL screening; MINUTE virus of mice; LABORATORY mice; BIOMATERIALS; DIAGNOSIS
- Publication
Laboratory Animals, 2013, Vol 47, Issue 2, p116
- ISSN
0023-6772
- Publication type
Article
- DOI
10.1177/0023677213478298