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- Title
Effects of rhAm on attachment, proliferation and immigration of human fibroblasts.
- Authors
LIN Zhi-kai; SHU Rong; CHENG Lan; SONG Zhong-chen; GUO Qiu-man; ZHANG Xiu-li
- Abstract
PURPOSE: To compare the effects of 25 kDa full-length rhAm and porcine EMPs on cell behaviors of human periodontal ligament fibroblasts (HPDLF) and foreskin fibroblasts(HFF). METHODS: rhAm was induced by BL21/pET28a-His-SUMO-rhAm express system, and 25 kDa full-length rhAm was analyzed by SDS-PAGE and Western blot. EMPs were extracted by acetic acid method. HPDLF and HFF were cultured in vitro. The cells were treated with rhAm and EMPs at different concentrations. The cell adhesion, proliferation and migration assays were qualitatively analyzed. The data was statistically analyzed with SAS 5.0 software package. RESULTS: 10-20 µg/mL rhAm significantly promoted the adhesion, proliferation and migration of HPDLF and HFF (P<0.05), but no significant difference between two proteins was found (P>0.05). CONCLUSIONS: 25 kDa rhAm and EMPs shows similar biological effects on fibroblast, which indicates that rhAm may play an important role in the periodontal regeneration through the activation of fibroblasts.
- Subjects
CELL proliferation; FIBROBLASTS; PERIODONTICS; FORESKIN; LABORATORY swine; ERYTHROCYTE membranes; CELL adhesion
- Publication
Shanghai Journal of Stomatology, 2013, Vol 22, Issue 1, p7
- ISSN
1006-7248
- Publication type
Article