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- Title
Anti-inflammatory properties of kaempferol via its inhibition of aldosterone signaling and aldosterone-induced gene expression.
- Authors
Liu, Zi-Kui; Xiao, Hong-Bo; Fang, Jun
- Abstract
Osteopontin (OPN), also called cytokine Eta-1, is a pro-inflammatory cytokine. Recent studies have shown that aldosterone increases OPN gene expression in endothelial cells. As a flavonoid compound, kaempferol has potent anti-inflammatory properties, but whether kaempferol regulates aldosterone signaling and aldosterone-induced gene expression is still unknown. Human umbilical vein endothelial cells (HUVECs) were pretreated with kaempferol (0, 1, 3, or 10 μmol/L) for 1 h prior to exposure to aldosterone (10−6 mol/L) for 24 h. Aldosterone induced generation of reactive oxygen species; OPN and cluster of differentiation 44 gene expression; phospho-p38 MAPK and NF-κB binding activity. The effect of aldosterone was abrogated by kaempferol and spironolactone (10−6 mol/L). The present results suggest that kaempferol exerts its anti-inflammatory properties via its inhibition of aldosterone signaling and aldosterone-induced gene expression in HUVECs.
- Subjects
OSTEOPONTIN; ALDOSTERONE synthesis; GENE expression; ENDOTHELIAL cells; SPIRONOLACTONE; NF-kappa B; CD44 antigen
- Publication
Canadian Journal of Physiology & Pharmacology, 2014, Vol 92, Issue 2, p117
- ISSN
0008-4212
- Publication type
Article
- DOI
10.1139/cjpp-2013-0298