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- Title
On the disruption of biochemical and biological assays by chemicals leaching from disposable laboratory plasticware.
- Authors
Olivieri, Aldo; Degenhardt, Owen S.; McDonald, G. Reid; Narang, Deepak; Paulsen, Isabelle M.; Kozuska, Janna L.; Holt, Andrew
- Abstract
Plastic consumables, used universally in bioscience laboratories, are presumed inert with respect to bioassay outcomes. However, it is clear that many pipette tips, microfuge tubes, and other plastic disposables leach bioactive compounds into assay solutions, profoundly affecting data and experimental interpretation. In this paper we discuss the nature and sources of leachates and review several examples of compromised bioassay data that speak to the probable widespread nature of this largely unrecognised source of error. Strategies for minimizing leachate interferences are discussed.
- Subjects
LEACHING; PLASTICS; BIOCHEMISTRY; BIOACTIVE compounds; BIOLOGICAL assay; SURFACE active agents; BISPHENOL A; ANTIBACTERIAL agents
- Publication
Canadian Journal of Physiology & Pharmacology, 2012, Vol 90, Issue 6, p697
- ISSN
0008-4212
- Publication type
Article
- DOI
10.1139/y2012-049