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- Title
Cyclosporin Increases Granulocyte/Macrophage Colony-Stimulating Factor (GM-CSF) Activity and Gene Expression in Murine Keratinocytes.
- Authors
Gallo, Richard L.; Grabbe, Stephan; Choi, Susanna S.; Bleicher, Paul; Granstein, Richard D.
- Abstract
Keratinocytes produce multiple cytokines in response to a variety of stimuli. The release of interleukin 1 (IL-1) from keratinocytes may be significant in intiation of cutaneous inflammation, and the presence of granulocyte/macrophage colony-stimulation factor (GM-CSF) is thought to be important in the regulation of antigen presenting function by epidermal Langerhans cells, Because cyclosporin inhibits interleukin 2 release from T cells, it has been suggested that cyclosporin may function as an anti inflammatory agent within the epidermis through inhibition of keratinocyte cytokine release. The investigation examined the direct effect of cyclosporin on the production of GM-CSF by murine keratinocytes and the keratinocyte cell line PAM 212. GM CSF bio activity increased in cell supernatants from keratinocytes exposed in vitro to 1 μ/ ml cyclosporin for up to 24h. GM-CSF and IL-1 mRNA levels in keratinocytes cultured under similar conditions or in the presence of lipopolysaccharide also increased. The lack of inhibition of GM-CSF expression following cyclosporin treatment is consistent with recent observations in T cells and is opposite to the effect of cyclosporin on interleukin.
- Subjects
CYCLOSPORINE; KERATINOCYTES; INTERLEUKIN-1; GRANULOCYTES; LEUCOCYTES; LANGERHANS cells
- Publication
Journal of Investigative Dermatology, 1992, Vol 98, Issue 3, p274
- ISSN
0022-202X
- Publication type
Article
- DOI
10.1111/1523-1747.ep12497858