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- Title
Effect of Recombinant Tumor Necrosis Factor-Alpha on Cultured Microvascular Endothelial Cells Derived From Human Dermis.
- Authors
Detmar, Michael; Imcke, Eckhart; Ruszczak, Zbigniew; Orfanos, Constantin E.
- Abstract
We investigated the effects of recombinant human tumor necrosis factor-alpha (TNF) on cell proliferation and on expression of MHC class II antigens and intercellular adhesion molecule ICAM-1 in human dermal microvascular endothelial cells (HDMEC) derived from human foreskin. Second- passage HDMEC were treated with 0.1-10,000 U/ml TNF for up to 6 d, and cell growth was assessed by cell counts and a recently developed fluorogenic assay using 4-methylumbelliferyl heptanoate as a substrate. APAAP immunocytochemistry was performed using monoclonal antibodies against HLA-DR, HLA-DQ, and ICAM-1. TNF did not markedly inhibit the growth of HDMEC but induced expression of HLA-DR (1,000 U/ml and more) and of ICAM-1 (1 U/ml and more). Combination with interferon-gamma led to synergistic ICAM-1 induction. These results demonstrate a profound effect of TNF on the activation of dermal microvascular endothelial cells and suggest a major role of TNF in the mediation of leucocyte adhesion to endothelial cells of the skin microvasculature with possible implications for the initiation and maintenance of inflammatory skin processes.
- Subjects
NECROSIS; DERMIS; MICROCIRCULATION disorders; CELL proliferation; IMMUNOGLOBULINS; IMMUNOCYTOCHEMISTRY
- Publication
Journal of Investigative Dermatology, 1990, Vol 95, p219S
- ISSN
0022-202X
- Publication type
Article
- DOI
10.1111/1523-1747.ep12875807