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- Title
Frequency and characterization of RHD variants in serologically D- Surinamese pregnant women and D- newborns.
- Authors
Zonneveld, Rens; Kanhai, Humphrey H.H.; Javadi, Ahmad; Veldhuisen, Barbera; Brand, Anneke; Zijlmans, Wilco C.W.R.; Schoot, C. Ellen; Schonewille, Henk; van der Schoot, C Ellen; Rhesus in Surinamese Neonates (RheSuN) Study Group
- Abstract
<bold>Background: </bold>Numerous RHD variant genes affect the expression of D on the red blood cell surface. In Suriname, 4.3% of pregnant women were D-, ranging from virtually zero to 7% among ethnic groups. Characterization of RHD variants, which are associated with a variable potential to induce anti-D, is of practical clinical importance especially in case of limited access to preventive measures. Here we report on the occurrence of RHD variant genes in Surinamese serologically D- pregnant women and their D- newborns from different ethnic groups.<bold>Study Design and Methods: </bold>The RheSuN study is a cross-sectional cohort study in D- pregnant women and their newborns, who visited hospitals in Paramaribo, Suriname, during routine pregnancy care. The presence of RHD variants was investigated using quantitative polymerase chain reaction targeting RHD Exons 5 and 7 and RH-multiplex ligation-dependent probe amplification.<bold>Results: </bold>Seven RHD variant genes were detected in 35 of 84 women and four RHD variant genes in 15 of 36 newborns. The RHD*03 N.01 and RHD*08 N.01 variants represented 87% of a total of 62 variant genes. Variants were comparably frequent among ethnicities. In four cases genotyping would have changed anti-D prophylaxis policy: one woman with a RHD*01EL.01 variant, not associated with anti-D formation and three D- newborns with RHD*09.01 and RHD*09.03.01 variants, potentially capable of inducing anti-D.<bold>Conclusion: </bold>RHD variants at risk for anti-D are common among serologic D- individuals from African descent in Suriname. While genotyping D- women has limited added value, it may be considered in newborns from D- women.
- Subjects
SURINAME; PREGNANT women; ETHNIC groups; ERYTHROCYTES; GENE expression; POLYMERASE chain reaction
- Publication
Transfusion, 2019, Vol 59, Issue 8, p2672
- ISSN
0041-1132
- Publication type
journal article
- DOI
10.1111/trf.15394