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- Title
Heparin in Clinical Doses 'Primes' Granulocytes to Subsequent Activation as Measured by Myeloperoxidase Release.
- Authors
Videm, V.
- Abstract
The author pre-incubated isolated human granulocytes with increasing doses of heparin (0-100 IU/mL) before subsequent stimulation with formyl-met-leu-phe. There was a dose-dependant increase in granulocyte activation as measured by myeloperoxidase release, quantitated in enzyme-immunoassay. This 'priming' effect of heparin was not due to activation of contaminating platelets or to endotoxin, and there was no reduction in granulocyte viability. Heparin-induced 'priming' of granulocytes may be clinically relevant in the setting of cardiopulmonary bypass. The author describes a sensitive and specific double antibody enzyme-immunoassay for myeloperoxidase from human granulocytes, with an interassay coefficient of variation of 12.6%, an intra-assay coefficient of variation of 4.3%, and a lower detection limit of 1.8 µg/l.
- Subjects
HEPARIN; GRANULOCYTES; IMMUNOASSAY; BLOOD platelets; ENDOTOXINS
- Publication
Scandinavian Journal of Immunology, 1996, Vol 43, Issue 4, p385
- ISSN
0300-9475
- Publication type
Article
- DOI
10.1046/j.1365-3083.1996.d01-57.x