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- Title
Gluten Stimulation of Coeliac Mucosa <em>In Vitro</em> Induces Activation (CD25) of Lamina Propria CD4<sup>+</sup> T cells and Macrophages but no Crypt-Cell Hyperplasia.
- Authors
Halstensen, T. S.; Scott, H.; Fausa, O.; Brandtzaeg, P.
- Abstract
Jejunal biopsy specimens from 10 patients with treated coeliac disease and seven non-coeliac controls were challenged in vitro with peptic-tryptic gluten digest. Mucosal T cells were examined in situ by three-colour immunofluorescence staining for expression of the activation marker CD25 (the p55 α-chain of interleukin-2 receptor) and the nuclear proliferation marker revealed by monoclonal antibody Ki-67. Intraepithelial T cells expressed CD25 rarely whereas the proportion of activated lamina propria T cells increased (P < 0.002) from median 2.8% (cultured with 20% fetal calf serum alone for 24-48 h) to 10.0% after 24 h with gluten (n = 10; range 1.1-17.4%) and to 10.4% after 48 h (n = 7; range 1.4-17.5%). Such gluten-induced increase of CD25+ T cells was not observed in specimens from non-coeliac control subjects. Crypt-cell hyperplasia and T-cell proliferation (Ki-67+) were observed neither in the coeliac nor in the control mucosae after gluten stimulation. Three-colour staining combining a polyclonal antibody reagent to CD3 and a monoclonal antibody to CD25 with a monoclonal antibody to CD45R0, CD4, CD8, the p75 β-chain of interleukin-2 receptor, integrin αEβ7, or HLA-DR showed that most of the CD25+ T cells (> 90%) were CD4+CD8-, co-expressed CD45R0 and the p75 β-chain, and often also the integrin αEβ7 but not HLA-DR. In addition to these activated T cells, a dominating population of CD25+ CD3- CD4+ subepithelial pan-HLA-class II+ macrophages (CD68+) with variable expression of the p75 β-chain was often induced by gluten challenge.
- Subjects
CELIAC disease; T cells; MACROPHAGES; HYPERPLASIA; IMMUNOFLUORESCENCE; MONOCLONAL antibodies
- Publication
Scandinavian Journal of Immunology, 1993, Vol 38, Issue 6, p581
- ISSN
0300-9475
- Publication type
Article
- DOI
10.1111/j.1365-3083.1993.tb03245.x