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- Title
Allorecognition of HLA DR2 and DR5 Molecules by V-Beta-8-Positive T-Cell Clones.
- Authors
Wilson, K. E.; Ball, E.; Stasny, P.; Capra, J. D.
- Abstract
V-beta-8-positive T cells were isolated from human peripheral blood lymphocytes using a monoclonal antibody specific for the Vβ8 family. Alloreactive T-cell lines were generated by stimulation with mononuclear cells from individuals homozygous for HLA DRI-DR9. Cloned Vβ8-positive T cells were then assayed for alloreactivity based on a proliferative assay using irradiated B-cell lines. Vβ8-positive T-cell clones alloreactive to DR2 and DR5 molecules were chosen for further study based on the association of these MHC antigens with autoimmune disease. DNA sequence analysis confirmed the use of the Vβ8 gene family as well as providing information on the use of the V, D, J and N segments in these alloreactive T-cell clones.
- Subjects
LYMPHOCYTES; IMMUNOGLOBULINS; CELL culture; LEUCOCYTES; MOLECULAR cloning; MONOCLONAL antibodies
- Publication
Scandinavian Journal of Immunology, 1991, Vol 33, Issue 2, p131
- ISSN
0300-9475
- Publication type
Article
- DOI
10.1111/j.1365-3083.1991.tb03743.x